7UDA

Structure of the EstG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	OTHER
Temperature [K]	277
Detector technology	PIXEL
Collection date	2021-05-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 41
Unit cell lengths	110.321, 110.321, 55.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 2.470
R-factor	0.182
Rwork	0.180
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ci8
RMSD bond length	0.009
RMSD bond angle	1.735
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.620	29.600	2.570
High resolution limit [Å]	2.470	8.920	2.470
Rmerge	0.108	0.047	0.781
Rmeas	0.117	0.052	0.847
Rpim	0.045	0.020	0.324
Total number of observations		3610	16345
Number of reflections	24085	554	2515
<I/σ(I)>	10.9	26.3	2.4
Completeness [%]	99.0	97.4	91.9
Redundancy	6.7	6.5	6.5
CC(1/2)	0.996	0.995	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	20% PEG500 MME, 10% PEG20000, 0.1 M Tris/Bicine pH 8.5 and 90 mM mixture of sodium nitrate, sodium phosphate dibasic and ammonium sulfate