7UD3
The Crystal Structure of Domain-Swapped Dimer Q108K:T51D:A28C:L36C:F57:W:W:W:R58 Mutant of hCRBPII with a Histidine Insertion in the Hinge Loop Region at 2.36 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-28 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | I 4 |
| Unit cell lengths | 140.652, 140.652, 63.956 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.850 - 2.370 |
| R-factor | 0.1927 |
| Rwork | 0.187 |
| R-free | 0.25770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.945 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.850 | 2.454 |
| High resolution limit [Å] | 2.369 | 2.369 |
| Rmerge | 0.088 | 0.701 |
| Number of reflections | 25561 | 2499 |
| <I/σ(I)> | 30.19 | 2.81 |
| Completeness [%] | 99.8 | 98.85 |
| Redundancy | 7.3 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 4000 PEG, Sodium acetate, ammonium acetate |
