7UCV
The Crystal Structure of Apo Domain-Swapped Dimer Q108K:T51D:A28CL36C R58:H:H:H:N59 HCRBPII with Histidine Insertion in the Hinge Loop Region at 2.19 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-24 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.952, 69.901, 107.254 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.950 - 2.190 |
R-factor | 0.2051 |
Rwork | 0.198 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rcq |
RMSD bond length | 0.007 |
RMSD bond angle | 0.872 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.950 | 2.230 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.075 | 0.264 |
Rmeas | 0.101 | 0.301 |
Number of reflections | 14150 | 1272 |
<I/σ(I)> | 4.2 | |
Completeness [%] | 94.8 | 85.2 |
Redundancy | 4.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, sodium acetate, ammonium acetate |