7UCV

The Crystal Structure of Apo Domain-Swapped Dimer Q108K:T51D:A28CL36C R58:H:H:H:N59 HCRBPII with Histidine Insertion in the Hinge Loop Region at 2.19 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	36.952, 69.901, 107.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.950 - 2.190
R-factor	0.2051
Rwork	0.198
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rcq
RMSD bond length	0.007
RMSD bond angle	0.872
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.950	2.230
High resolution limit [Å]	2.190	2.190
Rmerge	0.075	0.264
Rmeas	0.101	0.301
Number of reflections	14150	1272
<I/σ(I)>	4.2
Completeness [%]	94.8	85.2
Redundancy	4.2	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 4000, sodium acetate, ammonium acetate