7UCT
The Crystal Structure of Apo Domain-Swapped Dimer F57:H:H:H:H:H:H:R58 Mutant of HCRBPII with Histidine Insertion in the Hinge Loop Region at 2.5 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-03-20 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97633 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.797, 76.582, 110.525 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.290 - 2.520 |
R-factor | 0.1826 |
Rwork | 0.176 |
R-free | 0.23770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rcq |
RMSD bond length | 0.008 |
RMSD bond angle | 1.006 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.290 | 2.580 |
High resolution limit [Å] | 2.520 | 2.540 |
Rmerge | 0.090 | 0.458 |
Rmeas | 0.122 | 0.541 |
Number of reflections | 10638 | 947 |
<I/σ(I)> | 13.76 | 2.07 |
Completeness [%] | 99.2 | 98.5 |
Redundancy | 5.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, sodium acetate, ammonium acetate |