7UCQ
Pfs230 D1 domain in complex with 230AS-18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.220, 145.730, 78.270 |
Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
Resolution | 41.270 - 2.500 |
R-factor | 0.2058 |
Rwork | 0.204 |
R-free | 0.24840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jum |
RMSD bond length | 0.005 |
RMSD bond angle | 0.758 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.270 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 57254 | 3779 |
<I/σ(I)> | 8.21 | |
Completeness [%] | 97.7 | |
Redundancy | 3.4 | |
CC(1/2) | 0.990 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M sodium acetate, pH 4.6, 20% PEG 10000 |