7UCN

The Crystal Structure of Domain-Swapped Dimer Q108K:T51D:A28C:L36C:F57:H:R58 Mutant of hCRBPII with a Histidine Insertion in the Hinge Loop Region at 1.96 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-03
Detector	RAYONIX MX300HE
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	39.710, 56.580, 136.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.120 - 1.960
R-factor	0.2197
Rwork	0.215
R-free	0.26780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rcq
RMSD bond length	0.007
RMSD bond angle	0.872
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.120	2.035
High resolution limit [Å]	1.960	1.964
Rmeas	0.141
Number of reflections	22254	2048
<I/σ(I)>	33.3
Completeness [%]	98.8
Redundancy	13.3
CC(1/2)		0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 4000, ammonium acetate, sodium acetate