7UBX
Structure of a pore forming fragment of Clostridium difficile toxin A in complex with VHH AA6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.018, 85.995, 109.339 |
Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.650 | 1.880 |
High resolution limit [Å] | 1.810 | 1.810 |
Number of reflections | 88827 | 8321 |
<I/σ(I)> | 11.85 | |
Completeness [%] | 98.0 | |
Redundancy | 3.4 | |
CC(1/2) | 0.998 | 0.702 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Ammonium acetate, 0.1 M Bis-Tris, pH 5.7, 15% PEG 3350 |