7UBL
Transcription antitermination factor Qlambda in complex with Q-lambda-binding-element DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 131.102, 131.102, 49.545 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.352 - 2.177 |
| R-factor | 0.1914 |
| Rwork | 0.188 |
| R-free | 0.22850 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.352 | 2.220 |
| High resolution limit [Å] | 2.177 | 2.177 |
| Rmerge | 0.056 | 0.826 |
| Number of reflections | 23174 | 1143 |
| <I/σ(I)> | 26.7 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 0.1 M HEPES, pH 7, 1 M sodium citrate |
