7UBL

Transcription antitermination factor Qlambda in complex with Q-lambda-binding-element DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	131.102, 131.102, 49.545
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.352 - 2.177
R-factor	0.1914
Rwork	0.188
R-free	0.22850
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.352	2.220
High resolution limit [Å]	2.177	2.177
Rmerge	0.056	0.826
Number of reflections	23174	1143
<I/σ(I)>	26.7
Completeness [%]	99.5
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	0.1 M HEPES, pH 7, 1 M sodium citrate