7UBK

Transcription antitermination factor Qlambda, type-II crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	34.304, 64.317, 65.319
Unit cell angles	90.00, 98.35, 90.00

Refinement procedure

Resolution	45.588 - 1.970
R-factor	0.1888
Rwork	0.184
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mo1
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.000
High resolution limit [Å]	1.970	5.350	1.970
Rmerge	0.043	0.024	0.605
Rmeas	0.051	0.029	0.732
Rpim	0.028	0.016	0.405
Total number of observations	63986
Number of reflections	19614	1020	974
<I/σ(I)>	11.9
Completeness [%]	98.1	97.6	96.2
Redundancy	3.3	3.2	3
CC(1/2)		0.997	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	30% PEG600, 100 mM HEPES/sodium hydroxide, pH 7.5, 50 mM lithium sulfate, 10% glycerol