7UBJ

Transcription antitermination factor Qlambda, type-I crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 32
Unit cell lengths	54.341, 54.341, 110.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.273 - 1.460
R-factor	0.1783
Rwork	0.177
R-free	0.20260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mo1
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.490
High resolution limit [Å]	1.460	3.960	1.460
Rmerge	0.049	0.037	0.887
Rmeas	0.055	0.041	0.991
Rpim	0.024	0.018	0.438
Total number of observations	319426
Number of reflections	63011	3148	3116
<I/σ(I)>	11.9
Completeness [%]	99.7	99.1	100
Redundancy	5.1	5.2	5.1
CC(1/2)		0.996	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	20% v/v glycerol, 0.4 M potassium phosphate dibasic, 16% PEG8000