7UAT
The crystal structure of the K36A mutant of E. coli YGGS in complex with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-26 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.5406 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 124.010, 124.010, 83.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.575 - 2.000 |
| R-factor | 0.1932 |
| Rwork | 0.191 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w8g |
| Data reduction software | CrysalisPro (41.64.69a) |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.575 | 28.570 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.050 | 0.016 | 0.528 |
| Rmeas | 0.053 | 0.016 | 0.555 |
| Rpim | 0.016 | 0.005 | 0.169 |
| Total number of observations | 2646 | 16991 | |
| Number of reflections | 22247 | 285 | 1601 |
| <I/σ(I)> | 28 | 82.5 | 5.1 |
| Completeness [%] | 100.0 | 97 | 100 |
| Redundancy | 10.6 | 9.3 | 10.6 |
| CC(1/2) | 1.000 | 1.000 | 0.933 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, pH 7.5, 0.8M NaH2PO4/0.8M KH2PO4 |
