7U8T

Vps13 adaptor binding domain in complex with Mcp1 PxP motif peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	P 1 21 1
Unit cell lengths	83.735, 91.225, 132.769
Unit cell angles	90.00, 102.16, 90.00

Refinement procedure

Resolution	48.360 - 3.000
R-factor	0.2514
Rwork	0.250
R-free	0.28480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold2 model
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.360	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.044	0.322
Rmeas	0.052	0.378
Rpim	0.028	0.197
Number of reflections	39383	3799
<I/σ(I)>	17.2	3.4
Completeness [%]	98.5	97.6
Redundancy	3.5	3.6
CC(1/2)	0.999	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	295	75 mM imidazole pH 8.2, 70 mM lithium sulfate, 500 mM sodium chloride