7U8M
Crystal structure of chimeric hemagglutinin cH15/3 in complex with broad protective antibodies 31.a.83 and FluA-20
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0331 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 272.922, 155.729, 156.893 |
Unit cell angles | 90.00, 94.63, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.940 | 5.590 |
High resolution limit [Å] | 5.390 | 5.390 |
Rpim | 0.560 | 0.420 |
Number of reflections | 21811 | 1941 |
<I/σ(I)> | 8.9 | 1 |
Completeness [%] | 96.3 | 86.4 |
Redundancy | 3.4 | 2.4 |
CC(1/2) | 0.990 | 0.589 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium cacodylate, pH 6.5, 0.2 LiSO4, and 30% (w/v) polyethylene glycol 400 |