7U8L

Crystal structure of chimeric hemagglutinin cH15/3 in complex with broad protective antibody 31.a.83

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 3 2 1
Unit cell lengths	156.168, 156.168, 114.019
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.640 - 4.560
R-factor	0.3005
Rwork	0.300
R-free	0.30520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tg8 4we8 5kaq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.640	4.680
High resolution limit [Å]	4.560	4.560
Rpim	0.060	0.370
Number of reflections	9360	431
<I/σ(I)>	7.5	0.9
Completeness [%]	99.5	95.4
Redundancy	12.3	4.2
CC(1/2)	0.994	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris, pH 7.0, 40% (w/v) polyethylene glycol 300 and 5% (w/v) polyethylene glycol 1000