7U8J

Crystal structure of chimeric hemagglutinin cH4/3 in complex with broad protective antibody 31.a.83

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 3 2 1
Unit cell lengths	158.124, 158.124, 127.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.510 - 4.900
R-factor	0.3248
Rwork	0.324
R-free	0.34000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xl1 4we8 5kaq
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	5.070
High resolution limit [Å]	4.900	4.900
Rpim	0.050	0.300
Number of reflections	8670	550
<I/σ(I)>	15.3	1
Completeness [%]	99.7	98.2
Redundancy	14.6	10.8
CC(1/2)	0.996	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M imidazole pH 8.0 and 10% (w/v) polyethylene glycol 8,000