7U8D
FKBP12 mutant V55G bound to Rapa*-3Z
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-08-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 37.683, 44.138, 44.403 |
Unit cell angles | 112.14, 97.04, 104.57 |
Refinement procedure
Resolution | 36.600 - 1.390 |
R-factor | 0.1477 |
Rwork | 0.147 |
R-free | 0.17230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fkb |
RMSD bond length | 0.015 |
RMSD bond angle | 1.433 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.600 | 1.440 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.039 | 0.422 |
Rmeas | 0.046 | 0.496 |
Rpim | 0.024 | 0.259 |
Number of reflections | 46706 | 4275 |
<I/σ(I)> | 18.42 | 3.14 |
Completeness [%] | 93.2 | 85.18 |
Redundancy | 3.8 | 3.6 |
CC(1/2) | 0.999 | 0.825 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | sodium tartrate, PEG 3350 |