7U8B
Product of 14mer primer with activated asymmetric GA dimer diastereomer 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 99 |
| Detector technology | PIXEL |
| Collection date | 2022-02-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033167 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 43.455, 43.455, 81.122 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.162 - 1.730 |
| Rwork | 0.194 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c8o |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.965 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.093 | 0.499 |
| Rmeas | 0.103 | 0.554 |
| Rpim | 0.043 | 0.230 |
| Number of reflections | 9404 | 474 |
| <I/σ(I)> | 13.8 | 4.8 |
| Completeness [%] | 96.3 | 98.5 |
| Redundancy | 5 | 4.8 |
| CC(1/2) | 0.998 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 1.7 M Lithium sulfate, 50 mM HEPES pH 7.0, 50 mM Magnesium sulfate |
