7U8A

Product of 14mer primer with activated G monomer diastereomer 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	98
Detector technology	CCD
Collection date	2021-05-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 3 2 1
Unit cell lengths	42.873, 42.873, 82.763
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.416 - 2.100
Rwork	0.199
R-free	0.28180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c8o
RMSD bond length	0.038
RMSD bond angle	2.706
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.079	0.527
Rmeas	0.083	0.556
Rpim	0.026	0.175
Number of reflections	5553	273
<I/σ(I)>	25.9	4.2
Completeness [%]	99.9	100
Redundancy	10.2	9.9
CC(1/2)	0.993	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	50 mM PIPES pH 7.0, 65 mM Magnesium chloride, 1 mM Cobalt (III) Hexamine chloride, 26% v/v (+/-)-2-Methyl-2,4-pentanediol