7U84

Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 6.0 mM Mn2+ for 600s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-02-28
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9787
Spacegroup name	P 61
Unit cell lengths	97.854, 97.854, 81.545
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.950 - 1.710
R-factor	0.1885
Rwork	0.185
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.950	41.950	1.810
High resolution limit [Å]	1.710	5.090	1.710
Rmerge	0.070	0.035	1.534
Rmeas	0.077	0.039	1.694
Total number of observations	541269
Number of reflections	65683	3572	15010
<I/σ(I)>	11.88	40.05	1.01
Completeness [%]	99.3	99.9	97.3
Redundancy	5.743	5.687	5.574
CC(1/2)	0.999	0.999	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG2000 MME, 0.1 M MES