7U82

Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 1.0 mM Mn2+ for 600s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-02-28
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9787
Spacegroup name	P 61
Unit cell lengths	97.999, 97.999, 81.701
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.020 - 1.550
R-factor	0.1938
Rwork	0.192
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.020	42.020	1.640
High resolution limit [Å]	1.550	4.630	1.550
Rmerge	0.051	0.029	0.982
Rmeas	0.057	0.032	1.142
Total number of observations	679149
Number of reflections	65618	4771	19501
<I/σ(I)>	15.25	45.94	1.21
Completeness [%]	98.6	99.6	94.8
Redundancy	5.412	5.575	3.748
CC(1/2)	0.999	0.999	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG2000 MME, 0.1 M MES