7U81
Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 0.5 mM Mn2+ for 600s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-03-31 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9786 |
Spacegroup name | P 61 |
Unit cell lengths | 98.311, 98.311, 82.088 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.570 - 1.600 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.21940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.570 | 42.570 | 1.690 |
High resolution limit [Å] | 1.600 | 4.760 | 1.600 |
Rmerge | 0.052 | 0.024 | 1.225 |
Rmeas | 0.057 | 0.026 | 1.380 |
Total number of observations | 655592 | ||
Number of reflections | 65474 | 4416 | 18629 |
<I/σ(I)> | 17.29 | 61.7 | 1.1 |
Completeness [%] | 99.4 | 99.3 | 97.3 |
Redundancy | 5.585 | 5.671 | 4.671 |
CC(1/2) | 0.999 | 0.999 | 0.403 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG2000 MME, 0.1 M MES |