7U7Z
Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 0.12 mM Mn2+ for 600s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-03-31 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9786 |
Spacegroup name | P 61 |
Unit cell lengths | 98.215, 98.215, 82.157 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.770 - 1.670 |
R-factor | 0.1888 |
Rwork | 0.186 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.770 | 37.770 | 1.770 |
High resolution limit [Å] | 1.670 | 4.970 | 1.670 |
Rmerge | 0.057 | 0.020 | 1.371 |
Rmeas | 0.062 | 0.022 | 1.516 |
Total number of observations | 589069 | ||
Number of reflections | 103090 | 3880 | 16489 |
<I/σ(I)> | 16.29 | 61.26 | 1.11 |
Completeness [%] | 99.8 | 99.7 | 98.8 |
Redundancy | 5.714 | 5.703 | 5.491 |
CC(1/2) | 1.000 | 1.000 | 0.453 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG 2000MME, 0.1 M MES |