7U7X

Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 2.0 mM Mg2+ for 600s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-02-06
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.291, 98.291, 81.945
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.170 - 1.650
R-factor	0.1897
Rwork	0.189
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.170	32.170	1.750
High resolution limit [Å]	1.650	4.920	1.650
Rmerge	0.054	0.026	1.057
Rmeas	0.060	0.029	1.169
Total number of observations	609106
Number of reflections	106021	3960	16836
<I/σ(I)>	16.28	51.81	1.39
Completeness [%]	98.8	98.8	96.9
Redundancy	5.745	5.732	5.44
CC(1/2)	0.999	0.999	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES