7U7W
Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 1.0 mM Mg2+ for 600s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-02-06 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9786 |
Spacegroup name | P 61 |
Unit cell lengths | 97.969, 97.969, 81.761 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.420 - 1.660 |
R-factor | 0.1851 |
Rwork | 0.184 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.420 | 42.420 | 1.760 |
High resolution limit [Å] | 1.660 | 4.960 | 1.660 |
Rmerge | 0.050 | 0.020 | 1.048 |
Rmeas | 0.055 | 0.022 | 1.154 |
Total number of observations | 588160 | ||
Number of reflections | 101079 | 3860 | 15946 |
<I/σ(I)> | 18.85 | 66.43 | 1.46 |
Completeness [%] | 97.8 | 99.3 | 95.4 |
Redundancy | 5.819 | 5.725 | 5.634 |
CC(1/2) | 1.000 | 1.000 | 0.562 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG 2000MME, 0.1 M MES |