7U7V

Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 0.4 mM Mg2+ for 600s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-03-27
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.618, 98.618, 81.963
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.250 - 1.650
R-factor	0.1874
Rwork	0.186
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.250	42.250	1.750
High resolution limit [Å]	1.650	4.920	1.650
Rmerge	0.066	0.027	1.491
Rmeas	0.073	0.029	1.657
Total number of observations	607832
Number of reflections	54451	4036	17126
<I/σ(I)>	13.97	49.17	1.13
Completeness [%]	99.8	99.8	98.7
Redundancy	5.669	5.678	5.275
CC(1/2)	0.999	0.999	0.412

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES