7U7F
Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 10.0 mM Mn2+ for 90s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-12-14 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9787 |
Spacegroup name | P 61 |
Unit cell lengths | 98.269, 98.269, 81.983 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.550 - 1.650 |
R-factor | 0.2035 |
Rwork | 0.200 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kfh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.195 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.550 | 42.550 | 1.750 |
High resolution limit [Å] | 1.650 | 4.920 | 1.650 |
Rmerge | 0.058 | 0.023 | 1.312 |
Rmeas | 0.064 | 0.025 | 1.450 |
Total number of observations | 591871 | ||
Number of reflections | 104851 | 3986 | 17059 |
<I/σ(I)> | 15.75 | 58.61 | 1.13 |
Completeness [%] | 98.7 | 99.8 | 99.1 |
Redundancy | 5.645 | 5.645 | 5.484 |
CC(1/2) | 0.999 | 0.999 | 0.493 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG 2000MME, 0.1 M MES |