7U7D
Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 10.0 mM Mn2+ for 30s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-12-14 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9787 |
Spacegroup name | P 61 |
Unit cell lengths | 98.081, 98.081, 82.346 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.130 - 1.570 |
R-factor | 0.1844 |
Rwork | 0.182 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kfg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.106 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.130 | 42.130 | 1.660 |
High resolution limit [Å] | 1.570 | 4.680 | 1.570 |
Rmerge | 0.049 | 0.026 | 0.883 |
Rmeas | 0.054 | 0.029 | 1.018 |
Total number of observations | 672665 | ||
Number of reflections | 123528 | 4653 | 19793 |
<I/σ(I)> | 16.64 | 51.83 | 1.39 |
Completeness [%] | 99.6 | 99.6 | 98.4 |
Redundancy | 5.445 | 5.603 | 4.038 |
CC(1/2) | 0.999 | 0.999 | 0.522 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG 2000MME, 0.1 M MES |