7U76
Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 0.5 mM Mn2+ for 1800s then with 10 mM Mn2+ for 300s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-04-15 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9787 |
Spacegroup name | P 61 |
Unit cell lengths | 97.657, 97.657, 81.441 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.290 - 1.690 |
R-factor | 0.1853 |
Rwork | 0.182 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kff |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.290 | 42.290 | 1.640 |
High resolution limit [Å] | 1.550 | 4.620 | 1.550 |
Rmerge | 0.039 | 0.021 | 0.938 |
Rmeas | 0.043 | 0.023 | 1.094 |
Total number of observations | 693009 | ||
Number of reflections | 59674 | 4882 | 20642 |
<I/σ(I)> | 19.67 | 66.73 | 1.18 |
Completeness [%] | 99.7 | 99.7 | 98.2 |
Redundancy | 5.346 | 5.698 | 3.639 |
CC(1/2) | 1.000 | 0.999 | 0.484 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG 2000MME, 0.1 M MES |