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7U76

Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 0.5 mM Mn2+ for 1800s then with 10 mM Mn2+ for 300s

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-F
Synchrotron siteAPS
Beamline21-ID-F
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2021-04-15
DetectorMAR scanner 300 mm plate
Wavelength(s)0.9787
Spacegroup nameP 61
Unit cell lengths97.657, 97.657, 81.441
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution42.290 - 1.690
R-factor0.1853
Rwork0.182
R-free0.21920
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5kff
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwarePHENIX (1.19.2_4158)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]42.29042.2901.640
High resolution limit [Å]1.5504.6201.550
Rmerge0.0390.0210.938
Rmeas0.0430.0231.094
Total number of observations693009
Number of reflections59674488220642
<I/σ(I)>19.6766.731.18
Completeness [%]99.799.798.2
Redundancy5.3465.6983.639
CC(1/2)1.0000.9990.484
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP629810% PEG 2000MME, 0.1 M MES

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