7U75

Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 0.5 mM Mn2+ for 1800s then with 10 mM Mn2+ for 90s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-04-15
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.616, 98.616, 82.057
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.880 - 1.550
R-factor	0.1894
Rwork	0.187
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kff
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.880	37.880	1.640
High resolution limit [Å]	1.550	4.610	1.550
Rmerge	0.045	0.027	0.779
Rmeas	0.050	0.030	0.894
Total number of observations	692640
Number of reflections	65892	4888	19318
<I/σ(I)>	16.84	47.73	1.33
Completeness [%]	97.4	99.4	91.6
Redundancy	5.445	5.682	3.887
CC(1/2)	0.999	0.999	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES