7U73

Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 0.5 mM Mn2+ for 1800s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-12-14
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.152, 98.152, 82.103
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.500 - 1.560
R-factor	0.1828
Rwork	0.180
R-free	0.21320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kff
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.500	42.500	1.650
High resolution limit [Å]	1.560	4.650	1.560
Rmerge	0.061	0.026	1.480
Rmeas	0.064	0.027	1.586
Total number of observations	1361307
Number of reflections	64243	4763	20413
<I/σ(I)>	20.45	74.12	1.24
Completeness [%]	99.9	99.9	99.5
Redundancy	10.747	11.046	7.73
CC(1/2)	1.000	1.000	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES