7U71

Crystal Structure of Mouse Cadherin-23 EC13-15

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	27.898, 44.047, 133.954
Unit cell angles	90.00, 94.59, 90.00

Refinement procedure

Resolution	44.548 - 1.975
Rwork	0.175
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k8r 5wj8
RMSD bond length	0.011
RMSD bond angle	1.664
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.010
High resolution limit [Å]	1.975	1.975
Rmerge	0.099	0.209
Number of reflections	22984	1134
<I/σ(I)>	13.425	9.44
Completeness [%]	98.0	97.4
Redundancy	4.9	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1 M HEPES pH7.5 0.2 M NaOAc 20% PEG 3000