7U6U

Structure of an intellectual disability-associated ornithine decarboxylase variant G84R in complex with PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-06
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	74.147, 86.098, 153.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.049 - 1.850
R-factor	0.1822
Rwork	0.181
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s3f
RMSD bond length	0.005
RMSD bond angle	0.848
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.850	1.850
Number of reflections	84340	4377
<I/σ(I)>	11.4
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)	0.996	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M sodium acetate trihydrate pH 7.0, 20% w/v polyethylene glycol 3350