7U5D
I-F3b Cascade-TniQ full R-loop complex
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.52 |
Software | Warp,cryoSPARC,RELION,Coot,RosettaEM,PHENIX,Topaz,SerialEM,UCSF Chimera |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |