7U4Z

The ubiquitin-associated domain of human thirty-eight negative kinase-1 flexibly fused to the 1TEL crystallization chaperone via a 2-glycine linker and crystallized at very low protein concentration

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	67.375, 67.375, 58.683
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.690 - 2.030
R-factor	0.2034
Rwork	0.202
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qar 7tcy
RMSD bond length	0.005
RMSD bond angle	0.622
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.690	2.103
High resolution limit [Å]	2.030	2.030
Rmerge	0.220	1.842
Rmeas	0.226	1.890
Rpim	0.051	0.419
Number of reflections	8862	976
<I/σ(I)>	11.07	2.39
Completeness [%]	89.4	100
Redundancy	19.5	20.2
CC(1/2)	0.998	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	1.2 uL of 0.5 mg/mL protein combined with 1.2 uL of 100 mM Bis-Tris, pH 6.00, 630-750 mM Mg-Formate.