7U4Q
Human Synaptotagmin-1 C2A Y181F Ca2+ bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 81798 |
Spacegroup name | I 4 |
Unit cell lengths | 99.810, 99.810, 77.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.060 - 1.560 |
R-factor | 0.1648 |
Rwork | 0.164 |
R-free | 0.18880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wee |
RMSD bond length | 0.011 |
RMSD bond angle | 1.253 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.060 | 1.616 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.040 | 1.899 |
Rmeas | 0.042 | 2.013 |
Rpim | 0.011 | 0.649 |
Number of reflections | 53881 | 5347 |
<I/σ(I)> | 27.72 | 1.07 |
Completeness [%] | 99.9 | 99.63 |
Redundancy | 14.2 | 9.2 |
CC(1/2) | 1.000 | 0.434 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 100 mM HEPES, 2.3 M malonate |