7U3A

Structure of the Streptomyces venezuelae GlgX-c-di-GMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-12
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	129.470, 127.660, 179.720
Unit cell angles	90.00, 93.73, 90.00

Refinement procedure

Resolution	89.670 - 3.340
R-factor	0.2085
Rwork	0.205
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7u39
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.670	3.420
High resolution limit [Å]	3.340	3.340
Rpim	0.095	0.228
Number of reflections	42293	2854
<I/σ(I)>	6.2	1.9
Completeness [%]	99.5
Redundancy	2
CC(1/2)	0.977	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	40% ethylene glycol, 0.1 M sodium acetate pH 4.6