7U39

Structure of the apo form of Streptomyces venezuelae GlgX, the glycogen debranching enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	181.496, 204.856, 195.719
Unit cell angles	90.00, 90.43, 90.00

Refinement procedure

Resolution	67.920 - 3.510
R-factor	0.2164
Rwork	0.211
R-free	0.27560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vuy
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.920	3.550
High resolution limit [Å]	3.510	3.510
Rpim	0.012	0.363
Number of reflections	131795	4072
<I/σ(I)>	5.5	2.1
Completeness [%]	83.5
Redundancy	1.7
CC(1/2)	0.969	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	9% propanol, 675 mM ammonium citrate/ammonium hydroxide pH 8.5