7U2S
Structure of Paenibacillus xerothermodurans Apyc1 in the apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-02-11 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.030, 58.295, 76.188 |
Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
Resolution | 38.640 - 1.550 |
R-factor | 0.1733 |
Rwork | 0.173 |
R-free | 0.19290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7u2r |
RMSD bond length | 0.006 |
RMSD bond angle | 0.926 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.640 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rpim | 0.078 | 0.297 |
Number of reflections | 74391 | 3615 |
<I/σ(I)> | 5.4 | 2.3 |
Completeness [%] | 99.5 | 97.6 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES-KOH 7.5, 0.2 M Calcium Acetate, 10% PEG-8,000 |