7U2R
Structure of Paenibacillus sp. J14 Apyc1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-02-11 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 102.868, 102.868, 147.619 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.140 - 1.850 |
R-factor | 0.1778 |
Rwork | 0.177 |
R-free | 0.19980 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.177 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.140 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rpim | 0.038 | 0.666 |
Number of reflections | 39926 | 2401 |
<I/σ(I)> | 12.1 | 1.2 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 11.3 | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tric-HCl pH 8.5, 0.2 M MgCl2, 16% PEG-4000 |