7U1X
Structure of SPAC806.04c protein from fission yeast covalently bound to BeF3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-17 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.2822 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 59.300, 117.533, 153.435 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.920 - 2.120 |
R-factor | 0.1783 |
Rwork | 0.176 |
R-free | 0.21570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7t7k |
RMSD bond length | 0.008 |
RMSD bond angle | 0.873 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.920 | 2.170 |
High resolution limit [Å] | 2.110 | 2.110 |
Rpim | 0.057 | 0.378 |
Number of reflections | 61347 | 3701 |
<I/σ(I)> | 13 | 2 |
Completeness [%] | 98.6 | 82 |
Redundancy | 10 | 8.6 |
CC(1/2) | 0.997 | 0.592 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 1.8 M Sodium/potassium phosphate (pH 8.0), cryoprotected in crystallization solution supplemented with 25% sucrose |