7U1N
Crystal structure of the Anopheles darlingi AD-118 long form D7 salivary protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2020-08-12 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.730, 71.600, 71.750 |
| Unit cell angles | 90.00, 111.23, 90.00 |
Refinement procedure
| Resolution | 29.390 - 2.400 |
| R-factor | 0.2107 |
| Rwork | 0.206 |
| R-free | 0.26850 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.065 | 0.284 |
| Number of reflections | 24809 | 1239 |
| <I/σ(I)> | 15.77 | |
| Completeness [%] | 94.1 | 63.4 |
| Redundancy | 4.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 30%P EG 5000 MME, 0.2 M ammonium sulfate, 0.1M HEPES |
