7U1H
Crystal structure of Lens culinaris vicilin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.441, 92.504, 143.738 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 77.909 - 2.500 |
Rwork | 0.199 |
R-free | 0.28390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uik |
RMSD bond length | 0.008 |
RMSD bond angle | 1.677 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 143.740 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.144 | 0.782 |
Rmeas | 0.172 | 0.939 |
Rpim | 0.093 | 0.513 |
Number of reflections | 39945 | 4426 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 98.6 | |
Redundancy | 6.2 | 6.2 |
CC(1/2) | 0.991 | 0.812 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.01 M Zinc Chloride, 0.1 M Sodium acetate trihydrate, 15% PEG6000, with 20% glycerol as cryoprotectant |