7U1H

Crystal structure of Lens culinaris vicilin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	86.441, 92.504, 143.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.909 - 2.500
Rwork	0.199
R-free	0.28390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uik
RMSD bond length	0.008
RMSD bond angle	1.677
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	143.740	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.144	0.782
Rmeas	0.172	0.939
Rpim	0.093	0.513
Number of reflections	39945	4426
<I/σ(I)>	9.6
Completeness [%]	98.6
Redundancy	6.2	6.2
CC(1/2)	0.991	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.01 M Zinc Chloride, 0.1 M Sodium acetate trihydrate, 15% PEG6000, with 20% glycerol as cryoprotectant