7U0W

Structure of myxoma virus M062 protein variant MAV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 6
Unit cell lengths	148.844, 148.844, 38.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.422 - 3.205
R-factor	0.2897
Rwork	0.289
R-free	0.30720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cz3
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	128.900	3.420
High resolution limit [Å]	3.200	3.200
Rmerge	0.055	1.551
Rmeas	0.061	1.633
Rpim	0.020	0.508
Number of reflections		1441
<I/σ(I)>	31.9	1.5
Completeness [%]	99.7	98.4
Redundancy	9.7	10.2
CC(1/2)	0.999	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	100mM Imidazole, pH 8.0 11-14% PEG 3,350