7U0V
Structure of myxoma virus M062 protein variant Lau
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 64 |
Unit cell lengths | 94.827, 94.827, 44.136 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.410 - 2.450 |
R-factor | 0.2169 |
Rwork | 0.211 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cyw |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.123 | 2.528 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.054 | |
Rmeas | 0.057 | |
Rpim | 0.018 | |
Number of reflections | 846 | |
<I/σ(I)> | 23.92 | |
Completeness [%] | 99.5 | |
Redundancy | 9.8 | |
CC(1/2) | 0.990 | 0.407 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 50mM MgCl2 14% ethylene glycol 6% PEG8K 0.1M Tris pH 7.5 |