7U02
Structure of the C. crescentus DriD C-domain bound to ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.01 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 87.400, 87.400, 139.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 66.540 - 2.480 |
R-factor | 0.2338 |
Rwork | 0.230 |
R-free | 0.27900 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.600 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Rpim | 0.033 | 1.034 |
Number of reflections | 43538 | 1977 |
<I/σ(I)> | 15 | 0.7 |
Completeness [%] | 99.9 | |
Redundancy | 7.9 | |
CC(1/2) | 1.000 | 0.334 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | Ammonium sulphate |