7U01
Structure of CD148 fibronectin type III domain 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979276 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 155.068, 155.068, 47.837 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.711 - 2.297 |
R-factor | 0.21719288275 |
Rwork | 0.214 |
R-free | 0.26042 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.591 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.354 |
High resolution limit [Å] | 2.297 | 2.297 |
Rmerge | 0.098 | 1.100 |
Number of reflections | 26929 | 1712 |
<I/σ(I)> | 22.4 | |
Completeness [%] | 100.0 | |
Redundancy | 26.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M sodium acetate pH4.5, 3M NaCl |