7TZV
Structure of DriD C-domain bound to 9mer ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-02 |
Detector | DECTRIS EIGER2 S 4M |
Wavelength(s) | 1.01 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.023, 85.338, 93.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.490 - 1.650 |
R-factor | 0.1929 |
Rwork | 0.192 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DriD Cdomain p3121 form |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.100 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rpim | 0.029 | 0.978 |
Number of reflections | 55422 | 3432 |
<I/σ(I)> | 16.1 | 1.2 |
Completeness [%] | 98.1 | |
Redundancy | 5.9 | |
CC(1/2) | 0.999 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | 0.1 M MES pH 6.5, 0.1 M Imidazole, 20% PEG 8000 |