7TZH

Structure of human LAG3 domains 3-4 in complex with antibody single chain-variable fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	119.155, 78.521, 107.166
Unit cell angles	90.00, 111.44, 90.00

Refinement procedure

Resolution	46.480 - 2.430
R-factor	0.2146
Rwork	0.212
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsi
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.480	2.520
High resolution limit [Å]	2.430	2.430
Number of reflections	34666	3383
<I/σ(I)>	11.35
Completeness [%]	99.5	98.25
Redundancy	3.4
CC(1/2)	0.997	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	0.1 M HEPES pH 7.1, 13% PEG20000