7TZ2
Structure of human Fibrinogen-like protein 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-01 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 45.277, 110.893, 148.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.300 - 2.550 |
R-factor | 0.1871 |
Rwork | 0.185 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m7f |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 5.490 | 2.550 |
Rmerge | 0.349 | 0.180 | 1.347 |
Rmeas | 0.384 | 0.199 | 1.518 |
Rpim | 0.157 | 0.083 | 0.686 |
Total number of observations | 144191 | ||
Number of reflections | 25222 | 2723 | 2457 |
<I/σ(I)> | 3 | ||
Completeness [%] | 99.7 | 99.5 | 99.4 |
Redundancy | 5.7 | 5.7 | 4.5 |
CC(1/2) | 0.901 | 0.421 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1 M lithium chloride, 0.1 M citric acid pH 5.0 and 20% PEG 6000 |