7TXG

Structure of the Class II Fructose-1,6-Bisphosphatase from Francisella tularensis with native Mn++ divalent cation and partially occupied product F6P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 1
Unit cell lengths	64.176, 76.226, 77.924
Unit cell angles	68.02, 68.22, 76.65

Refinement procedure

Resolution	19.940 - 1.900
R-factor	0.1887
Rwork	0.187
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7js3
RMSD bond length	0.008
RMSD bond angle	0.915
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.054
Rmeas	0.077	0.208
Rpim	0.054	0.150
Number of reflections	85208	8144
<I/σ(I)>	25.27	4.6
Completeness [%]	85.9	82.5
Redundancy	1.8	1.8
CC(1/2)	0.996	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	8% Tacsimate pH 6.0 and 20% Polyethylene glycol 3350